|
KMID : 1189420090220010062
|
|
Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2009 Volume.22 No. 1 p.62 ~ p.75
|
|
|
The effects of Gamisipjeontang-gamibang on injured tissue after burn elicitation
|
|
Kim Hee-Young
Hong Seung-Ug
|
|
|
Abstract
|
|
|
Objective : The purpose of this study is to examine the effects of Gamisipjeontang-gamibang(GT) on an experimental burn elicitation.
Method : The absorbance of the photo cell mixed with GT at the Abs 560 ㎚ was measured after irradiation for 1 min. In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase(SOD) activity was measured. Also, in order to know effects on the skin regeneration in the burned mice, we counted the eosinophil in blood from animals via cardiac puncture and observed the histological structure in the epidermal basal layer and the dermal section on the 3rd, 7th and 14th day after burn elicitation. We also studied changes in angiogenesis in the capillary surrounding the basal layer and dermal papilla. The changes of HSP70 distribution and changes of p53 positive reaction were observed to investigate the changes of the stress in the skin as well.
Result : The results indicated that GT has a significant impact on the antioxidant effect on skin cells of mice after burn elicitation by increasing SOD activity in vitro test. GT seems to decrease MIP-2 which induces neutrophil infiltration and promotes the angiogenesis dose-dependently. Furthermore, GT decreased HSP70, the expression of which was induced by elevated temperatures, and p53 which induce a apoptosis after stress.
Conclusion : GT can be applied to burned skin through its antioxidant effect and skin regeneration property.
|
|
|
KEYWORD
|
|
|
Burned ski, SOD activity, HSP70, p53, Gamisipjeon-tang
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|